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Séminaire de Jean-François Berret

Particles and nanowires in interaction with living cells : from fundamentals to applications

J.-F. Berret

Matiére et Systèmes Complexes, UMR 7057 CNRS Université Denis Diderot Paris-VII, Batiment Condorcet, 10 Rue Alice Domon et Léonie Duquet, F-75205 Paris, France

When disperse in biological fluids (e.g. plasma, lung fluid), engineered particles are coated with proteins, resulting in the formation of a protein corona. It was suggested that the protein corona is critical in regulating the conditions of entry into the cytoplasm of living cells. Recent reports describe this phenomenon as ubiquitous and independent of the particle. In a first part, I discuss the concept of protein corona for nanometer size particles and show that under controlled surface chemistry, protein corona and endocytosis can be prevented [1,2]. As a result, particles are stealth in vivo and display remarkable biodistribution and imaging properties [3]. Freestanding nanowires of a few microns long exhibit in contrast different interactions with cells. The internalization process is likely to be macropinocytosis, the wires being dispersed in the cytosol. In a second part, I evaluate the applications of magnetic wires as probes for cell biomechanics [4]. I show that the viscoelastic properties of the intracellular medium can be accurately determined and that important questions on the nature of this fluid can be answered.

[1] N. Ould-Moussa, M. Safi, M.-A. Guedaud-Boudeville, D. Montero, H. Conjeaud and J.-F. Berret, In vitro toxicity of nanoceria : effect of coating and stability in biofluids , Nanotoxicology 8, 799 – 811 (2014)
[2] V. Torrisi, A. Graillot, L. Vitorazi, Q. Crouzet, G. Marletta, C. Loubat, and J.-F. Berret, Preventing corona effects : multi-phosphonic acid poly(ethylene glycol) copolymers for stable stealth iron oxide nanoparticles, Biomacromolecules (2014)
[3] Q. L. Vuong, J.-F. Berret, J. Fresnais, Y. Gossuin and O. Sandre, A Universal Scaling Law to Predict the Efficiency of Magnetic Nanoparticles as MRI T2-Contrast Agents, Advanced Health. Materials 1:502-512 (2012)
[4] L. Chevry, R. Colin, B. Abou and J.-F. Berret, Intracellular microrheology probed by micron-sized wires, Biomaterials 34 (2013) 6299 - 6305


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