BEGIN:VCALENDAR VERSION:2.0 PRODID:-//SPIP/Plugin Agenda//NONSGML v1.0//FR X-WR-TIMEZONE:Europe/Paris CALSCALE:GREGORIAN X-WR-CALNAME;VALUE=TEXT:Séminaire de Damien Baigl -- Laboratoire de Physique des Solides - UMR 8502 X-WR-RELCALID: BEGIN:VEVENT SUMMARY:Séminaire de Damien Baigl UID:20150323T095622-a2537-e6@ DTSTAMP:20150323T095622 DTSTART:20150327T110000 DTEND:20150327T123000 CREATED:20150323T095622 LAST-MODIFIED:20150323T095621 ORGANIZER;CN=ABECASSIS[] ATTENDEE:Damien Baigl DESCRIPTION:he triggerable and programmable softness of synthetic biological (or not) systems : from genes to coffee rings We exploit intrinsic soft matter properties of biological systems (DNA\, proteins\, membranes) to regulate their functions in vitro as well as we implement programmable biological functions inside synthetic soft matter systems. For instance\, we can stimulate genomic DNA molecules at the intramolecular level using microfluidic interface positioning [1]. We also use light-induced nucleic acid conformational changes [2\,3] to control gene expression at both transcription and translation levels [4\,5]. This is applied for the in vitro regulation of various proteins\, including fluorescent proteins [4\,5] and enzymes [6]. We can also place a protein under chemical or optical control by direct conjugation to giant DNA [7]. At a supramolecular level\, we engineer well-defined cell models in the form of giant liposomes\, which are functionalized with triggerable membrane proteins by direct cell-free reconstitution [8]. At micro- to macro-scales\, we develop laser-free methods where a simple light stimulus (e.g.\, from a LED device) is used to manipulate [9]\, divide [10]\, and mix [11] either continuous flows (light-driven microfluidics) or discrete droplets (digital optofluidics [12\, 13])\, with high spatio-temporal resolution in a cost-effective\, portable\, and robust manner. Finally\, we use light to direct the deposition of colloids from an evaporative droplet\, thus placing the so called coffee ring effect under optical control. [14\,15] References : [1] N. K. Mani et al.\, Chem. Commun. 49\, 6858 (2013) [2] A. Diguet et al.\, Chem. Eur. J. 16\, 11890 (2010) [3] A. Venancio-Marques et al.\, ACS Nano 8\, 3654–3663 (2014) [4] A. Estévez-Torres et al.\, Proc. Natl. Acad. Sci. USA 106\, 12219 (2009) [5] S. Rudiuk et al.\, Biomacromolecules 12\, 3945 (2011) [6] A. Venancio-Marques et al.\, ACS Synth. Biol. 1\, 526 (2012) [7] S. Rudiuk et al.\, Angew. Chem. Int. Ed. 51\, 12694 (2012) [8] Y.-J. Liu et al.\, ChemBioChem 14\, 2243 (2013) [9] A. Diguet et al.\, Angew. Chem. Int. Ed. 48\, 9281 (2009) [10] A. Diguet et al.\, Lab Chip 11\, 2666 (2011) [11] A. Venancio-Marques et al.\, J. Am. Chem. Soc. 135\, 3218 (2013) [12] D. Baigl\, Lab Chip 12\, 3637 (2012) [13] A. Venancio-Marques\, D. Baigl\, Langmuir 30\, 4207–4212 (2014) [14] M. Anyfantakis\, D. Baigl\, Angew. Chem. Int. Ed. 53\, 14077–14081 (2014) [15] M. Anyfantais et al.\, Langmuir\, in press (2015) CATEGORIES:Séminaire de Damien Baigl URL: SEQUENCE:0 STATUS:CONFIRMED END:VEVENT END:VCALENDAR